The Dutch Cryo-EM School will take place from 27 June to 02 July 2022 in Leiden and Delft. The aim of this school is to teach the basic principles and practical aspects of image processing to structural and molecular biologists wishing to determine macromolecular structures by cryoEM. The course will cover all aspects of cryoEM structure determination, ranging from sample preparation over data collection to processing and interpretation of high-resolution image data, for both single particle images and tomograms, and will be aimed at advanced PhD students and postdocs using cryoEM for structural analysis.
Application and registration:
The event is limited up to 20 participants. For selection purposes, please note that your application will not be considered without motivation.
During registration, please provide a brief motivation (max. 300 words) including a short description of your project, your level of prior experience with cryo-EM/ET and how you and your project will benefit from this school. You will also need to provide the name of your PI. The results will be announced approximately 2-3 weeks after the application deadline on 02 May 2022.
Registration fees:
There are no registration fees for participants from academia. The program includes lunch/coffee on all course days, and the course dinner on Wednesday 29 June. Transportation to and from the venues, and accommodation, are not included in the school and need to be arranged by the participants.
10:45- 12:00ThermoFisherScientific – Energy filters and detectors (chair: Ludo Renault)
12:00- 13:00Lunch breakUniversiteit Leiden - Gorlaeus Building – DM 0.13
13:00- 14:00JEOL – Introduction to Electron Optics (chair: Ludo Renault)Universiteit Leiden - Gorlaeus Building – DM 0.13
14:00- 15:30Microscope alignment and session setup (chair: Ludo Renault)Universiteit Leiden - Gorlaeus Building - NeCEN
15:30- 16:00Breaking questions (chair: Ludo Renault)Universiteit Leiden - Gorlaeus Building - EM 1.19
16:00- 18:00Microscope alignment and session setup (chair: Ludo Renault)Universiteit Leiden - Gorlaeus Building - NeCEN
Tuesday, 28th of June
09:50- 10:00Ludo Renault – Session introduction (chair: Ludo Renault)
10:00- 11:00CryoSol – Introduction to cryo-EM sample preparation (chair: Ludo Renault)Universiteit Leiden - Gorlaeus Building - EM 1.09
11:00- 11:15Coffee breakUniversiteit Leiden - Gorlaeus Building - EM 1.09
11:15- 12:15Wim Hagen (EMBL) – Best practices of microscopy for data collection for SPA (chair: Ludo Renault)Universiteit Leiden - Gorlaeus Building - EM 1.09
12:15- 13:00Lunch breakUniversiteit Leiden - Gorlaeus Building - EM 1.09
13:00- 15:30SPA data colllection (chair: Ludo Renault)Universiteit Leiden - Gorlaeus Building - NeCEN
15:30- 16:00Breaking questions (chair: Arjen Jakobi)Universiteit Leiden - Gorlaeus Building - EM 1.21
16:00- 18:00SPA data collection (chair: Ludo Renault)Universiteit Leiden - Gorlaeus Building - NeCEN
Wednesday, 29th of June
09:50- 10:00Tzviya Zeev Ben-Mordehai – Session Introduction (chair: Tzviya Zeev Ben-Mordehai)Universiteit Leiden - Gorlaeus Building - EM 1.09
10:00- 11:00Kay Grünewald (CSSB) – Introduction to Electron Cryo-Tomography (chair: Tzviya Zeev Ben-Mordehai)Universiteit Leiden - Gorlaeus Building - EM 1.09
11:00- 11:15Coffee breakUniversiteit Leiden - Gorlaeus Building - EM 1.09
11:15- 12:15Wim Hagen (EMBL) – Best practices for Tomography Data Collection (chair: Tzviya Zeev Ben-Mordehai)Universiteit Leiden - Gorlaeus Building - EM 1.09
12:15- 13:15Kay Grünewald (CSSB) – Correlative Light and Electron Microscopy (chair: Tzviya Zeev Ben-Mordehai)Universiteit Leiden - Gorlaeus Building - EM 1.09
13:15- 14:00Lunch breakUniversiteit Leiden - Gorlaeus Building - EM 1.09
14:00- 15:30Tomography data collection (chair: Ludo Renault)Universiteit Leiden - Gorlaeus Building - NeCEN
15:30- 16:00Breaking questions (chair: Tzviya Zeev Ben-Mordehai)Universiteit Leiden - Gorlaeus Building - EM 1.21
16:00- 18:00Tomography data collection (chair: Ludo Renault)Universiteit Leiden - Gorlaeus Building - NeCEN
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